Fig. 7. Abundance of F. heteroclitus 14-3-3 protein in gill epithelium of fish acclimated to either SW or for 24h or 48h to FW. (A) Example of a western blot developed with an antibody against a synthetic peptide that is 100% conserved in the N terminus of 14-3-3.a. This antibody recognizes two bands with apparent molecular mass of 28kDa and 29kDa. The abundance of both 14-3-3 bands increases after transfer of fish from SW to FW. (B) Quantification of 14-3-3 in gill epithelium of F. heteroclitus acclimated to SW and after transfer to FW. DVU, densitometric volume units; values are means ± S.E.M.; N=4. An asterisk indicates a significant difference to the respective 14-3-3 band in the SW sample (P<0.05).