Fig. 4. Inhibition of acid secretion by bafilomycin A₁ and Sch-28080. Urinary bladders were mounted in vitro in chambers as described in Materials and methods and the legend to Fig. 1. When the short-circuit current was stable, bafilomycin A₁ was introduced into the mucosal solution to the indicated final concentration. When the short-circuit current again stabilized, Sch-28080 was then added to the mucosal solution to the concentration indicated. Bars show inhibition by each compound as a percentage of baseline acid secretion (-4.7±1.25 µA (per area of chamber, 1.43 cm²), N=7). ^*Significant effect of treatment (P<0.01).