Fig. 7. Western blot of salt/ethanol wash proteins showing a band reactive with antiserum against a glycophosphatidylinositol (GPI)-anchored folate receptor and reactive with anti-cross-reactive-determinant antiserum. Lane 1, treated with antifolate-binding-protein antiserum. The protein band at the arrow has an approximate molecular mass of 37 kDa. Lane 2, treated with anti-cross-reactive-determinant antiserum. Note that a band at the same molecular mass, 37 kDa, is recognized. Gels contained 12% polyacrylamide.