JEB -- PAQUETTE et al. 204 (16): 2899 Figure IG2

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Fig. 2. Proteins removed from pellicle by phospholipase C (PLC) treatment at 37 and 0°C. (A) Western blot probed with anti-A and anti-B antisera, Lane 1, supernatant of sham-treated pellicle at 0°C; lane 2, supernatant of PLC-treated pellicle at 0°C; lane 3, supernatant of sham-treated pellicle at 37°C; lane 4, supernatant of PLC-treated pellicle at 37°C. (B) Same supernatants as in A but probed with anti-cross-reacting-determinant (anti-CRD) antiserum. The filled arrow points to surface antigens; the open arrow points to proteins of 40-60 kDa in gradient gels ranging from 6 to 12 % polyacrylamide.