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Fig. 1. Phospholipase treatment removes proteins with glycophosphatidylinositol (GPI) anchors from Paramecium pellicles. (A) Western blot probed with anti-A and anti-B antisera. Lane 1, pellet of sham-treated pellicle; lane 2, supernatant of sham-treated pellicle; lane 3, pellet of inositol-phosphate-specific phospholipase C (PLC)-treated pellicle; lane 4, supernatant of PLC-treated pellicle. The filled arrow points to surface antigens. The open arrow points to 40-60 kDa proteins. (B) Western blot probed with anti-cross-reacting-determinant (anti-CRD) antiserum, which binds to the cleaved GPI anchor. Lanes as in A. Gradient gels contained 8 % polyacrylamide.