Fig. 7. Expression of the crustacean cardioactive peptide (CCAP) transcript in the terminal ganglion of Manduca sexta. (A) Day 2 second-instar larva. (B) Air-filled fourth-instar larva. (C) Day 3 fifth-instar larva. (D) Wandering day 3 fifth-instar larva. (E) Day 10 pupa. (F) Day 0 adult. (A-D) Terminal ganglia of larvae are formed by the fusion of the seventh abdominal ganglion (denoted as A7) and abdominal ganglia 8-10 (denoted as A8). Lateral cells, concave arrows; anterior cells, convex arrows; A8 cells started to express the CCAP transcript after the head capsule stage of a fourth-instar larva, concave arrowheads; two pairs of cells that express the CCAP transcript after wandering, convex arrowheads. (E,F) Terminal ganglia of late pupae (pupal day 14) and adults (adult day 0) are formed by the fusion of the sixth abdominal ganglion (denoted A6) with A7 and A8-A10. CCAP-labeled cells in A6, convex arrowheads; in A7 cells, concave arrows; in A8-A10 cells, arrowheads. Scale bars, 50 µm.