Fig. 6. Expression of the crustacean cardioactive peptide (CCAP) transcript in the abdominal ganglia (A1-A6) of Manduca sexta. (A) Cells labeled with the in situ probe in the first abdominal ganglion (A1) from a day 2 second-instar larva. (B) Labeled cells in A2 of a day 1 fifth-instar larva. (C) Labeled cells in A2 of a day 3 fifth-instar larva. (D) Labeled cells in A4 of a day 0 pupa (P0). (E) Labeled cells in the posterior portion (T3, A1 and A2) of the fused thoracic ganglion from a day 10 pupa (P10). (F) Labeled cells in A3 of a P10 pupa. (G-I) Three different planes of focus of A3 in a day 0 adult, from ventral (G) to dorsal (I). (A-I) Lateral cells, concave arrows; anterior cells, convex arrows; midline cells, arrows; ventral unpaired neurons, arrowheads. Scale bars, 50 µm.