Fig. 3. Expression of the crustacean cardioactive peptide (CCAP) transcript in the subesophageal ganglion (SEG) of Manduca sexta. (A) Ventral view of an air-filled third-instar SEG showing a pair of posteriolaterally located cells (concave arrows) and a pair of smaller midline cells (arrows). (B) Ventral view of an air-filled fourth-instar larval SEG showing the previously discussed four cells (described in A) and an anteriolateral pair (convex arrows; left cell out of focus). (C) Ventral view of an SEG from a day 0 fourth-instar larva showing the posteriolaterally located cells (concave arrows), the anteriolateral cells (convex arrows) and a single anterior cell to the left of the midline (arrowhead). (D) Posterior view of an adult SEG showing only one pair of CCAP-expressing cells (arrows). Inset: diagram of the adult brain showing the area from where D was obtained (box; X indicates positions of cells). Scale bars, 50 µm.