Fig. 3. Follicles from ovaries of malaria-infected Anopheles stephensi developing normally (left) or showing evidence of apoptosis (right). (A) Whole-mounts of individual follicles 24h post-bloodfeeding, viewed under ultraviolet light after staining with Acridine Orange. The follicle in the left-hand frame (Ai) is exhibiting autofluorescence, and condensed apoptotic nuclei are visible in the follicle on the right, stained orange (a) (Aii). Scale bars, 20 µm. (B) Sections of follicles from ovaries 16h post-bloodfeeding stained with Methyl Green and treated by TUNEL (see Materials and methods). DNA fragmentation (indicated by brown staining) can be observed in follicular epithelial (f) and nurse (n) cells in the right-hand frame (Bii). Scale bars, 10 µm. (C) Electron micrographs of a normal follicle 18 h post-bloodfeeding (Ci) and a resorbing follicle 22h post-bloodfeeding (Cii). Patency (p) has developed in follicular epithelial cells of the normal follicle, and yolk spheres (y) have been deposited, whereas in the resorbing follicle no yolk is visible, epithelial cells are not patent and their nuclei contain condensed chromatin (c). Scale bars, 2 µm.