Fig.6. Thermal stability of the ATPase activity of Gillichthys mirabilis Hsc70. Native Hsc70 was incubated in assay buffer at 50, 60, 62.5, 65 and 80°C for 40min. During the 40min incubation period, 2µg samples were taken in triplicate every 10min, combined with 3.7x10⁵Bq of [-³²P]ATP in 50µl of assay buffer and placed in a water bath at 23°C. The amount of ATP hydrolysis was measured after 15min by thin-layer chromatography. Values are mean pixel absorbance volume (background-corrected) ± S.D. for N=3 samples for each time point. See Materials and methods for full a description of the assay buffer components.