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Fig. 1. Schematic diagram of the CCD microspectrophotometer. BI, back illumination; BIR, infrared filter; BS, beam splitter; CCD, charge-coupled device camera; CCS, custom computer software; CRO, reflective objective as condenser; FO, fibre optic cable; M, microscope; O, objective lens; PREP, preparation; S, xenon lamp (light source); SH, shutter; SIR, infrared filter mounted on a swing arm; SP, spectrograph; VA, X–Y variable aperture; XYS, X–Y stage.