Fig. 7. Levels of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF-2K/F-2,6BPase), pyruvate kinase (PK), fructose-1,6-bisphosphatase (FBPase) and glucose transporter type 2 (Glut2) gene expressions in the liver of food-deprived fish and of fish fed with 20% carbohydrates (+CHO) or without carbohydrates (-CHO). (A) Glut2, (B) PK, (C) 6PF-2K/F-2,6BPase, (D) FBPase. For each gene, two different northern blots (loading with fish fed 6h and 24h after feeding) were done; an analysis by densitometry of mRNA levels (arbitrary units) for five fish from each treatment group weighted by 16S rRNA values was performed (Visio-Mic II software). The results are expressed as means ± S.D. (N=5). Significant differences within groups are represented by different letters (Tukey’s test, P<0.05).