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Fig. 3. Partial cloning of the 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (6PF-2K/F-2,6BPase) gene in rainbow trout. (A) nucleotide and deduced amino acid sequences of the rainbow trout 6PF-2K/F-2,6BPase clone. Underlined letters correspond to the primer sequences. (B) Alignments of the partial amino acid deduced sequence from 6PF-2K/F-2,6BPase cDNA of the rainbow trout (T_6PF-2K/F-2,6BPase) (GenBank accession number: AF246148) with human 6PF-2K/F-2,6BPase (H_6PF-2K/F-2,6BPase) (GenBank accession number: P16168) and gilthead seabream (Sparus aurata) 6PF-2K/F-2,6BPase (SB_6PF-2K/F-2,6BPase) (GenBank accession number: U84724). Underlined letters correspond to the primer sequences. Asterisks mark amino acid residues homologous between 6PF-2K/F-2,6BPases. Residues marked by an arrow are key residues for the 6PF-2K/F-2,6BPase (Meton et al., 1999a).