Fig. 8. Confocal microscopy confirms that p26 translocates into nuclei in response to heat shock, anoxia and low pH. Nuclei were double stained using Alexa-Fluor 488 (Ai–Di), and DNA was detected using using Bopro-3 (Aii–Dii). Nuclei were isolated from control embryos (A), embryos heat-shocked from 22 to 50°C (B), embryos after 2 weeks of anoxia (C) and embryos homogenized and incubated at pH6.5 (D). The scale bar in D applies to all parts of the figure.