Fig. 3. Effect of heat shock and recovery on rates of transcription and subcellular localization of p26. Nuclear run-on assays measured transcription rates in nuclei isolated from embryos subjected to heat shock. Conditions included 22°C (control), 22–42°C, 22–46°C, 22–50°C and also 22–50°C followed by 2 or 4h of recovery at 25°C. Nuclei were then isolated and assayed as described in Materials and methods. Transcription data were normalized to control nuclei, and all assays were performed at pH8.0. Levels of p26 were obtained by immunoblotting and densitometry, and represent nuclear p26 as a percentage of the total. Error bars are +1 S.D., based on three separate experiments.