Fig. 2. Effect of heat shock on nuclear localization of p26 and transcription. (A) Coomassie-Blue-stained gel of proteins from nuclei isolated from control (C) and heat-shocked (HS) embryos (22–50°C) used in 15min mock run-on assays at pH8.0. Samples of nuclei were prepared for SDS–PAGE at both initiation (C0, HS0) and termination (C15, HS15) of the mock assay. Loading was equalized at 1x106 nuclei per lane, and the arrow indicates p26. (B) Western immunoblotting of the samples in A treated with antibody to detect p26. (C) Transcription rates of nuclei from control and heat-shocked embryos. Run-on assays were carried out as described in Materials and methods, at the pH indicated. Error bars are +1 S.D. based on three separate experiments.