Fig. 1. Effects of heat shock on subcellular localization of p26 and final hatching levels. (A) Embryos were subjected to increasing temperatures (from 22°C to 50°C at 0.7°Cmin^-1) and sampled at 22, 42, 46 and 50°C. Supernatant (S) and pellet (P) fractions were prepared for SDS–PAGE as described in Materials and methods. The arrow indicates p26 location on this Coomassie-Blue-stained gel. The positions of marker proteins (kDa) are shown. (B) Embryos from the experiment shown in A were also used in hatching assays or analyzed by western immunoblotting. The percentage of total p26 present in the pellet (filled circles) was determined by densitometry of western blots. Open circles indicate the percentage of larvae that hatched. Data points represent the means of three replicate experiments ± S.D.; error bars that are not visible are contained within the symbols.