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Fig. 1. Schematic diagram (not to scale) showing the self-referencing ion-selective (SeRIS) microelectrode for the study of K+ transport by isolated Malpighian tubules. The individual segments of the Malpighian tubule and their relationship to the ureter are shown. At all sites, the SeRIS microelectrode was vibrated over a distance of 100µm near the basolateral surface of the tubule.