Fig. 5. Transcripts for the two FK506-binding proteins FKB1_SUBDO and FKB2_SUBDO from Suberites domuncula, identified using northern blotting. (A) Determination of the size of the two transcripts, SUBDOFKB1 (FKB1; 0.62kb) and SUBDOFKB2 (FKB2; 0.79kb); the positions of size markers (kb) are given on the left. (B) RNA from transplants was obtained as described in Materials and methods. (i) Autografts (not treated with FK506), (ii) allografts (untreated) and (iii) allografts (treated with 20ng ml^-1 of FK506) were taken, RNA was extracted, and northern blots were performed. RNA was extracted from the graft zones, 0 (lane a), 10 (lane b) or 12h (lane c) after the start of the transplantation experiments. The intensities of the bands corresponding to SUBDOFKB1 (FKB1) and SUBDOFKB2 (FKB2) were standardized against those seen with ß-tubulin, as described in Materials and methods. (iv) In parallel, RNA from allografts (treated with 20ng ml^-1 FK506) were size-separated and analyzed with the S. domuncula ß-tubulin probe SDBTUB.