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Fig. 4. (A) Levels of the 9.0kb trkB (the high-affinity neurotrophin receptor of brain-derived neurotrophic factor) transcript in cultured prehierarchal follicle granulosa cells following overnight pre-culture and subsequent treatment for 18h without (control) or with vasoactive intestinal peptide (VIP; 1µmoll-1) or follicle-stimulating hormone (FSH; 100ngml-1) in the absence or presence of the phosphodiesterase inhibitor IBMX (0.01µmoll-1). (B) Levels of trkB mRNA in cultured preovulatory follicle granulosa cells following treatment for 18h without or with VIP or luteinizing hormone (LH) in the absence or presence of 3-isobutyl-1-methylxanthine (IBMX). trkB mRNA was standardized to 18S rRNA and expressed as fold difference compared with non-cultured (T0) cells. {ddagger}P<0.05 compared with T0 by a post-hoc paired t-test; *P< 0.05 compared with control; §P<0.05 compared with the respective treatment in the absence of IBMX. Values are means + S.E.M. (N=3 replicate experiments).