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Fig. 4. Chloride cell surface area in yolk-ball preparations incubated for 48h in fresh water (FW) and sea water (SW) supplemented with cortisol. Values are expressed as means ± S.E.M. (N=8 samples). Two-way ANOVA showed a significant effect of salinity (between FW and SW, P<0.0001), but no significant effect of the dose of cortisol (P=0.2982).