Fig. 6. Proteins present in control conditions and 1 and 3h after a 30min 37.5°C heat shock in salivary glands of OR25 larvae and of T32 strain larvae acclimated for 3 days at 25°C, as detected by silver staining and immunoblotting of two-dimensional electrophoresis gels. (A,B) Silver staining of the Hsp70 region in OR25 and T32 strains, respectively. 70c, Hsp70 cognates. (C,E) Hsp70 family members, as detected by antibody 7.10, in OR25 and T32 strains, respectively. Note the difference in Hsp68 (68) accumulation in the two strains and in the protein positioned immediately underneath the major Hsp70 spot (low MW). (D,F) Actin, as detected by silver staining, in OR25 and T32 strains, respectively.